Plant Sex Test
How do I collect samples for the Plant Sex Test?
What scientific test do you use?
Why do you want me to use cotyledon leaves?
Can Phylos come out and collect samples for me?
Can I bring my plants to Phylos for collection?
PHYLOS CERTIFIED GENOTYPE TEST
What do I get with a Phylos Certified test?
When will I receive my results?
How do you know which samples are clones?
What is genetic variation?
Why do two samples that are clones have slightly different levels of genetic variation (heterozygosity)?
How do clones differ from siblings in an inbred line?
How did the population groups get their names?
Plant Sex Test
The Plant Sex Test is available in multiples of 4 starting at $59.80 per kit. Results are included in the cost of the test.
Yes! Please call us for details on bulk pricing.
No. Please return the card with 1 blank quadrant. We do not offer refunds for unused tests. Never tamper with the card by cutting it.
You can order on our website by creating an account at testing.phylosbioscience.com. You can also visit one of our retail partners in your region.
No. As long as you keep your cards clean and dry they will last for multiple years.
PRO TIP: Buy cards before you pop seeds! This allows you to test at the earliest stage possible, saving you maximum time, money, space, and resources.
Read the instruction card included in your kit carefully before beginning to collect samples. Contact us at 503-206-6599 or email@example.com for questions about collecting samples.
Back at the lab, we extract DNA from your sample collection cards and use qPCR analysis to identify the Y chromosome. All cannabis plants have an XX or XY combination, indicating a female or male plant at a genetic level.
There are a number of reasons. 1) Taking a sample from a cotyledon leaf (the first rounded leaf) is the fastest way to identify the gender of your seedlings. 2) There is no THC in cotyledon leaves making samples legal to send in the mail. Our tests were designed to be in compliance with the Controlled Substances Act.
***If your cotyledon leaves are wilting or have already fallen off, please call us.
No. You must purchase kits and prepare your samples following the instructions included in your kits. For large orders, Phylos will offer training to your staff including how to organize your plants for sampling, and best practices for collection, shipping, and viewing results. Call us at 503-206-6599 to place a large order and schedule a training session.
No. We do not accept any plant material at our facility. All samples must be prepared using our collection kits prior to submission.
PRO TIP: Always keep plant stakes with plants until harvest to ensure quality control.
Please mail to: 2611 SW 3rd Ave, Ste 224, Portland OR 97201
Yes. We designed our collection methods to be legal to send across state lines. Our kits are designed to collect a DNA smear from cotyledon leaves, which means there’s no THC and no plant tissue.
Schedule a sample shipment within your Phylos account using a tracking number. This lets us know your samples will be arriving soon, and allows us to send you results on time. Our lab is closed on weekends, so plan for your samples to arrive on a Monday or Tuesday to avoid weekend gaps.
No. The price of the test includes the sample collection kit, testing, and results.
Results will be available within two business days of the lab receiving your samples. During our peak season (March-May), results may be delayed due to high volume. You can track the status of your order within your Phylos account or call us at 503-206-6599.
You can access results and track the status of orders within your Phylos account.
No. The Plant Sex Test and Phylos Certified Genotype Test use different processing methods and have seperate sample collection kits.
GENERAL QUESTIONS AND PRO TIPS
Our test saves growers time and money because we can identify plant sex just 10 days after germination, before transplanting. No need for a flip room or to spend resources on unwanted plants. Plus, it removes the risk of an unnoticed male plant accidentally pollinating your entire crop.
No. It is widely accepted that plants “flip” when exposed to environmental stresses, such as high temperatures, light leaks, and nutrient deficiencies. Always keep a close eye on your plants and keep plant stakes with your plants until flowering for quality control purposes.
Remove the plant immediately. If you think you may have mixed up samples, contact us at firstname.lastname@example.org or 503-206-6599 to inquire about a retest. Always keep plant stakes with your plants until harvest.
Phylos Certified Genotype Test
$295 per kit. Contact us for bulk pricing!
One. Each kit contains what you need to submit a sample for a single plant variety.
We need 0.5g sample of thin, dried stem material. If using fresh stem, we strongly recommend doubling the weight 1g as the material will lose weight as it dries. Do not send flower.
Your stem sample should be placed in the envelope included in your sample collection kit and can be sent using USPS or FedEX. Do not return samples in the plastic washing tube.
You will receive a Phylos Certified seal, which is a unique identification for your variety of cannabis. You will also receive a full report that includes information about relatives, genetic variation, and heritage. The Galaxy is a 3D visualization of the cannabis world as we know it, which you can view online by clicking here.
Based on our testing schedule, you will receive results approximately 4 weeks after sending in your sample. Please contact us at email@example.com or 503-206-6599 for an estimated timelines of results.
We use genetic similarity to determine whether two samples are clones. Genetic similarity tells us how alike two samples are by comparing their DNA and counting the differences between them. Samples must be at least 99% genetically identical to be considered clones.
Cannabis, like humans, has two similar copies of every part of their DNA. When you read the DNA sequence, the two copies have slight differences between them. Genetic variation (or heterozygosity), within one variety is the amount of difference between the two copies.
Two clones could have slightly different levels of genetic variation for a couple of reasons. First, one sample could be missing a few data points that are in the other sample. Second, there is technical variation introduced during processing of the sample, which can contribute slight differences in otherwise identical samples. Third, if two clonal lines were separated a long time ago, they can have what is called somaclonal variation. As the plants grow, their cells divide and during every cell division the DNA is copied. These copies are almost identical, but some errors happen during the copying process, which leads to slight differences between clonal lines.
Inbreeding reduces genetic variation and increases trait persistence. If a line is inbred, the siblings will have very low levels of genetic variation. Most cloning is in lines with higher levels of genetic variation. Cloning preserves the genetic variation and therefore clones can have high levels of genetic variation, but will be identical to each other.
We named groups by the varieties that are dominant with the group – the names are based on phenotype or a representative variety name. Skunk and OG Kush are two group names because they are older, well-known varieties that form distinct clusters. We have a group named CBD because many of the varieties in there have higher levels of CBD (e.g., Cannatonic, AC/DC). We named a group Berry because there were many varieties with “berry” in their names that formed this group. Hemp also forms a distinct cluster. Finally, we have a group called “Landrace” because many of the samples are not known to be drug cultivars and were collected from natural populations. However, there are likely other landrace samples that would not be part of this cluster – we just haven’t sampled them yet!
Anyone can go to the URL on a Phylos Certified seal and access all of the information available for that plant. This includes a genetic report and company profile.
All Phylos Certified Genotype Tests include a time-stamped report. Additionally, all submitters may choose to make their data public with the Open Cannabis Project — an organization working to preserve the diversity of cannabis and fight false patent attempts. Both are considered forms of defensive IP.
Phylos Certified tests alone do not make it possible to obtain a patent. That said, a time-stamped report can supplement a plant patent application filed by the creator of a unique plant.
No. There is currently no expiration for a Phylos Certified variety.